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Endpoints","\u002Fconnector-development\u002Ftutorial\u002Fsila-endpoints\u002F","20.connector-development\u002F20.tutorial\u002F10.sila-endpoints",{"title":836,"path":837,"stem":838,"type":46,"device":6},"Data Endpoints","\u002Fconnector-development\u002Ftutorial\u002Fdata-endpoints\u002F","20.connector-development\u002F20.tutorial\u002F20.data-endpoints",{"title":840,"path":841,"stem":842,"type":46,"device":6},"Controls","\u002Fconnector-development\u002Ftutorial\u002Fcontrols\u002F","20.connector-development\u002F20.tutorial\u002F30.controls",{"title":844,"path":845,"stem":846,"type":46,"device":6},"SiLA Data Types","\u002Fconnector-development\u002Ftutorial\u002Fdata-types\u002F","20.connector-development\u002F20.tutorial\u002F40.data-types",{"title":848,"path":849,"stem":850,"type":46,"device":6},"Feature","\u002Fconnector-development\u002Ftutorial\u002Fconnector-structure\u002F","20.connector-development\u002F20.tutorial\u002F70.connector-structure",{"title":852,"path":853,"stem":854,"type":46,"device":6},"Hardware Communication","\u002Fconnector-development\u002Ftutorial\u002Fhardware-communication\u002F","20.connector-development\u002F20.tutorial\u002F90.hardware-communication",{"title":272,"type":6,"device":6,"path":856,"stem":857,"children":858,"page":41},"\u002Fconnector-development\u002Fconcepts\u002F","20.connector-development\u002F30.concepts",[859],{"title":860,"path":861,"stem":862,"type":285,"device":6},"SiLA","\u002Fconnector-development\u002Fconcepts\u002Fsila\u002F","20.connector-development\u002F30.concepts\u002Fsila",{"title":864,"path":865,"stem":866,"children":867,"page":41},"Guides","\u002Fconnector-development\u002Fguides\u002F","20.connector-development\u002F40.guides",[868,872,876,880,884,888,892,895,899],{"title":869,"path":870,"stem":871,"type":24,"device":6},"Configuration","\u002Fconnector-development\u002Fguides\u002Fconfiguration\u002F","20.connector-development\u002F40.guides\u002F20.configuration",{"title":873,"path":874,"stem":875,"type":24,"device":6},"Logging","\u002Fconnector-development\u002Fguides\u002Flogging\u002F","20.connector-development\u002F40.guides\u002F21.logging",{"title":877,"path":878,"stem":879,"type":24,"device":6},"Deployment","\u002Fconnector-development\u002Fguides\u002Fdeployment\u002F","20.connector-development\u002F40.guides\u002F30.deployment",{"title":881,"path":882,"stem":883,"type":24,"device":6},"Security","\u002Fconnector-development\u002Fguides\u002Fsecurity\u002F","20.connector-development\u002F40.guides\u002F40.security",{"title":885,"path":886,"stem":887,"type":24,"device":6},"Subscriptions","\u002Fconnector-development\u002Fguides\u002Fsubscriptions\u002F","20.connector-development\u002F40.guides\u002F45.subscriptions",{"title":889,"path":890,"stem":891,"type":24,"device":6},"Execution Modes","\u002Fconnector-development\u002Fguides\u002Fexecution-modes\u002F","20.connector-development\u002F40.guides\u002F46.execution-modes",{"title":380,"path":893,"stem":894,"type":24,"device":6},"\u002Fconnector-development\u002Fguides\u002Ferror-handling\u002F","20.connector-development\u002F40.guides\u002F50.error-handling",{"title":896,"path":897,"stem":898,"type":24,"device":6},"Serial Troubleshooting","\u002Fconnector-development\u002Fguides\u002Fserial-troubleshooting\u002F","20.connector-development\u002F40.guides\u002F70.serial-troubleshooting",{"title":900,"path":901,"stem":902,"type":24,"device":6},"Testing","\u002Fconnector-development\u002Fguides\u002Ftesting\u002F","20.connector-development\u002F40.guides\u002F80.testing",{"title":904,"path":905,"stem":906,"children":907,"type":6,"device":6},"References","\u002Fconnector-development\u002Freference\u002F","20.connector-development\u002F50.reference\u002Findex",[908],{"title":904,"path":905,"stem":906,"type":200,"device":6},{"id":910,"title":364,"body":911,"description":2469,"device":6,"extension":2470,"links":6,"meta":2471,"navigation":981,"path":2472,"seo":2473,"stem":717,"type":24,"__hash__":2474},"docs\u002F06.liquid-handling-and-robots\u002F43.agilent-bravo\u002F25.liquid-classes.md",{"type":912,"value":913,"toc":2459},"minimark",[914,918,924,935,940,948,1084,1089,1094,1152,1156,1163,1211,1223,1227,1230,1233,1241,1248,1252,1255,1496,1500,1507,1831,1842,1884,1888,1898,2428,2445,2455],[915,916,917],"p",{},"Liquid classes define the motion parameters and volume correction curves used during aspirate and dispense operations. The SDK provides predefined liquid classes for common configurations and supports creating custom liquid classes for specialized protocols.",[915,919,920],{},[921,922,923],"strong",{},"Prerequisites",[925,926,927],"ul",{},[928,929,930,931,934],"li",{},"Basic understanding of the Bravo pipetting operations (See ",[932,933,617],"a",{"href":713},")",[936,937,939],"h2",{"id":938},"predefined-liquid-classes","Predefined Liquid Classes",[915,941,942,943,947],{},"The SDK includes predefined liquid classes for different head types, tip sizes, and volume ranges. Use the ",[944,945,946],"code",{},"BravoLiquidClasses"," factory to access them.",[949,950,955],"pre",{"className":951,"code":952,"language":953,"meta":954,"style":954},"language-python shiki shiki-themes github-light-high-contrast github-light-high-contrast github-dark-default","from unitelabs.labware.agilent import BravoLiquidClasses\n\nliquid_classes = BravoLiquidClasses\n\n# Access a predefined class by name\nliquid_class = liquid_classes.OQ_96LT_water_highVol\n\n# Use with aspirate\nawait bravo.pipette_head.aspirate(\n    plate=source_reservoir,\n    volume=100,\n    liquid_class=liquid_class,\n)\n","python","",[944,956,957,976,983,994,999,1006,1021,1026,1032,1041,1053,1067,1078],{"__ignoreMap":954},[958,959,962,966,970,973],"span",{"class":960,"line":961},"line",1,[958,963,965],{"class":964},"sxkbv","from",[958,967,969],{"class":968},"sc-9e"," unitelabs.labware.agilent ",[958,971,972],{"class":964},"import",[958,974,975],{"class":968}," BravoLiquidClasses\n",[958,977,979],{"class":960,"line":978},2,[958,980,982],{"emptyLinePlaceholder":981},true,"\n",[958,984,986,989,992],{"class":960,"line":985},3,[958,987,988],{"class":968},"liquid_classes ",[958,990,991],{"class":964},"=",[958,993,975],{"class":968},[958,995,997],{"class":960,"line":996},4,[958,998,982],{"emptyLinePlaceholder":981},[958,1000,1002],{"class":960,"line":1001},5,[958,1003,1005],{"class":1004},"sIsDC","# Access a predefined class by name\n",[958,1007,1009,1012,1014,1017],{"class":960,"line":1008},6,[958,1010,1011],{"class":968},"liquid_class ",[958,1013,991],{"class":964},[958,1015,1016],{"class":968}," liquid_classes.",[958,1018,1020],{"class":1019},"sOiAX","OQ_96LT_water_highVol\n",[958,1022,1024],{"class":960,"line":1023},7,[958,1025,982],{"emptyLinePlaceholder":981},[958,1027,1029],{"class":960,"line":1028},8,[958,1030,1031],{"class":1004},"# Use with aspirate\n",[958,1033,1035,1038],{"class":960,"line":1034},9,[958,1036,1037],{"class":964},"await",[958,1039,1040],{"class":968}," bravo.pipette_head.aspirate(\n",[958,1042,1044,1048,1050],{"class":960,"line":1043},10,[958,1045,1047],{"class":1046},"sNqAu","    plate",[958,1049,991],{"class":964},[958,1051,1052],{"class":968},"source_reservoir,\n",[958,1054,1056,1059,1061,1064],{"class":960,"line":1055},11,[958,1057,1058],{"class":1046},"    volume",[958,1060,991],{"class":964},[958,1062,1063],{"class":1019},"100",[958,1065,1066],{"class":968},",\n",[958,1068,1070,1073,1075],{"class":960,"line":1069},12,[958,1071,1072],{"class":1046},"    liquid_class",[958,1074,991],{"class":964},[958,1076,1077],{"class":968},"liquid_class,\n",[958,1079,1081],{"class":960,"line":1080},13,[958,1082,1083],{"class":968},")\n",[1085,1086,1088],"h3",{"id":1087},"available-liquid-classes","Available Liquid Classes",[1090,1091,1093],"h4",{"id":1092},"_96lt-head","96LT Head",[1095,1096,1097,1116],"table",{},[1098,1099,1100],"thead",{},[1101,1102,1103,1107,1110,1113],"tr",{},[1104,1105,1106],"th",{},"Liquid Class",[1104,1108,1109],{},"Tip",[1104,1111,1112],{},"Volume Range",[1104,1114,1115],{},"Description",[1117,1118,1119,1136],"tbody",{},[1101,1120,1121,1127,1130,1133],{},[1122,1123,1124],"td",{},[944,1125,1126],{},"OQ_96LT_water_lowVol",[1122,1128,1129],{},"AgilentTip_200",[1122,1131,1132],{},"0–50 µL",[1122,1134,1135],{},"Water, low volume",[1101,1137,1138,1143,1146,1149],{},[1122,1139,1140],{},[944,1141,1142],{},"OQ_96LT_water_highVol",[1122,1144,1145],{},"AgilentTip_250",[1122,1147,1148],{},"51–250 µL",[1122,1150,1151],{},"Water, high volume",[1085,1153,1155],{"id":1154},"finding-liquid-classes","Finding Liquid Classes",[915,1157,1158,1159,1162],{},"Use the ",[944,1160,1161],{},"find()"," method to discover liquid classes matching specific criteria.",[949,1164,1166],{"className":951,"code":1165,"language":953,"meta":954,"style":954},"from unitelabs.labware.agilent.tips import AgilentTip_250\n\nresults = liquid_classes.find(tip=AgilentTip_250, volume=100)\n",[944,1167,1168,1180,1184],{"__ignoreMap":954},[958,1169,1170,1172,1175,1177],{"class":960,"line":961},[958,1171,965],{"class":964},[958,1173,1174],{"class":968}," unitelabs.labware.agilent.tips ",[958,1176,972],{"class":964},[958,1178,1179],{"class":968}," AgilentTip_250\n",[958,1181,1182],{"class":960,"line":978},[958,1183,982],{"emptyLinePlaceholder":981},[958,1185,1186,1189,1191,1194,1197,1199,1202,1205,1207,1209],{"class":960,"line":985},[958,1187,1188],{"class":968},"results ",[958,1190,991],{"class":964},[958,1192,1193],{"class":968}," liquid_classes.find(",[958,1195,1196],{"class":1046},"tip",[958,1198,991],{"class":964},[958,1200,1201],{"class":968},"AgilentTip_250, ",[958,1203,1204],{"class":1046},"volume",[958,1206,991],{"class":964},[958,1208,1063],{"class":1019},[958,1210,1083],{"class":968},[1212,1213,1216],"callout",{"color":1214,"icon":1215},"info","i-heroicons-information-circle",[915,1217,1218,1219,1222],{},"When no ",[944,1220,1221],{},"liquid_class"," is provided to aspirate or dispense, the SDK automatically selects a matching liquid class based on the mounted tip type and the requested volume.",[936,1224,1226],{"id":1225},"volume-correction","Volume Correction",[915,1228,1229],{},"Liquid classes define polynomial coefficients that correct for systematic pipetting errors. The corrected volume determines the actual plunger (W-axis) position during aspirate and dispense operations.",[915,1231,1232],{},"The correction formula is a polynomial:",[949,1234,1239],{"className":1235,"code":1237,"language":1238},[1236],"language-text","corrected = c₀ + c₁ × volume + c₂ × volume² + ...\n","text",[944,1240,1237],{"__ignoreMap":954},[915,1242,1243,1244,1247],{},"For example, a liquid class with coefficients ",[944,1245,1246],{},"[0.0, 1.0074]"," applies a linear correction: the plunger moves slightly further than the nominal volume to compensate for dead volume or compression effects.",[936,1249,1251],{"id":1250},"liquid-class-parameters","Liquid Class Parameters",[915,1253,1254],{},"Every liquid class defines the following parameters that control motion during pipetting:",[1095,1256,1257,1269],{},[1098,1258,1259],{},[1101,1260,1261,1264,1267],{},[1104,1262,1263],{},"Parameter",[1104,1265,1266],{},"Default",[1104,1268,1115],{},[1117,1270,1271,1284,1295,1312,1327,1340,1353,1366,1378,1391,1403,1416,1428,1440,1451,1462,1473,1484],{},[1101,1272,1273,1278,1281],{},[1122,1274,1275],{},[944,1276,1277],{},"liquid",[1122,1279,1280],{},"Mixture()",[1122,1282,1283],{},"Liquid type (e.g., water, ethanol)",[1101,1285,1286,1290,1292],{},[1122,1287,1288],{},[944,1289,1196],{},[1122,1291,1129],{},[1122,1293,1294],{},"Compatible tip type",[1101,1296,1297,1306,1309],{},[1122,1298,1299,1302,1303],{},[944,1300,1301],{},"min_volume"," \u002F ",[944,1304,1305],{},"max_volume",[1122,1307,1308],{},"0 \u002F 250 µL",[1122,1310,1311],{},"Valid volume range",[1101,1313,1314,1319,1324],{},[1122,1315,1316],{},[944,1317,1318],{},"coefficients",[1122,1320,1321],{},[958,1322,1323],{},"0.0, 1.0",[1122,1325,1326],{},"Polynomial volume correction coefficients",[1101,1328,1329,1334,1337],{},[1122,1330,1331],{},[944,1332,1333],{},"aspirate_velocity",[1122,1335,1336],{},"5.0 mm\u002Fs",[1122,1338,1339],{},"Plunger velocity during aspirate",[1101,1341,1342,1347,1350],{},[1122,1343,1344],{},[944,1345,1346],{},"aspirate_acceleration",[1122,1348,1349],{},"10.0 mm\u002Fs²",[1122,1351,1352],{},"Plunger acceleration during aspirate",[1101,1354,1355,1360,1363],{},[1122,1356,1357],{},[944,1358,1359],{},"aspirate_velocity_into_wells",[1122,1361,1362],{},"50.0 mm\u002Fs",[1122,1364,1365],{},"Z descent velocity into wells",[1101,1367,1368,1373,1375],{},[1122,1369,1370],{},[944,1371,1372],{},"aspirate_velocity_out_of_wells",[1122,1374,1362],{},[1122,1376,1377],{},"Z retract velocity out of wells",[1101,1379,1380,1385,1388],{},[1122,1381,1382],{},[944,1383,1384],{},"aspirate_acceleration_into_wells",[1122,1386,1387],{},"100.0 mm\u002Fs²",[1122,1389,1390],{},"Z descent acceleration",[1101,1392,1393,1398,1400],{},[1122,1394,1395],{},[944,1396,1397],{},"aspirate_acceleration_out_of_wells",[1122,1399,1387],{},[1122,1401,1402],{},"Z retract acceleration",[1101,1404,1405,1410,1413],{},[1122,1406,1407],{},[944,1408,1409],{},"aspirate_post_delay_ms",[1122,1411,1412],{},"250 ms",[1122,1414,1415],{},"Delay after aspirate completes",[1101,1417,1418,1423,1425],{},[1122,1419,1420],{},[944,1421,1422],{},"dispense_velocity",[1122,1424,1336],{},[1122,1426,1427],{},"Plunger velocity during dispense",[1101,1429,1430,1435,1437],{},[1122,1431,1432],{},[944,1433,1434],{},"dispense_acceleration",[1122,1436,1349],{},[1122,1438,1439],{},"Plunger acceleration during dispense",[1101,1441,1442,1447,1449],{},[1122,1443,1444],{},[944,1445,1446],{},"dispense_velocity_into_wells",[1122,1448,1362],{},[1122,1450,1365],{},[1101,1452,1453,1458,1460],{},[1122,1454,1455],{},[944,1456,1457],{},"dispense_velocity_out_of_wells",[1122,1459,1362],{},[1122,1461,1377],{},[1101,1463,1464,1469,1471],{},[1122,1465,1466],{},[944,1467,1468],{},"dispense_acceleration_into_wells",[1122,1470,1387],{},[1122,1472,1390],{},[1101,1474,1475,1480,1482],{},[1122,1476,1477],{},[944,1478,1479],{},"dispense_acceleration_out_of_wells",[1122,1481,1387],{},[1122,1483,1402],{},[1101,1485,1486,1491,1493],{},[1122,1487,1488],{},[944,1489,1490],{},"dispense_post_delay_ms",[1122,1492,1412],{},[1122,1494,1495],{},"Delay after dispense completes",[936,1497,1499],{"id":1498},"custom-liquid-class-with-coefficients","Custom Liquid Class with Coefficients",[915,1501,1502,1503,1506],{},"Create a custom liquid class by subclassing ",[944,1504,1505],{},"BravoLiquidClass"," and providing direct polynomial coefficients. This approach is best when you already know the correction formula.",[949,1508,1510],{"className":951,"code":1509,"language":953,"meta":954,"style":954},"import dataclasses\nimport decimal\n\nfrom unitelabs.labware.agilent.liquids.bravo_liquid_class import BravoLiquidClass\nfrom unitelabs.labware.agilent.tips import AgilentTip_250, AgilentTip\nfrom unitelabs.labware.liquids import Mixture, PredefinedLiquids\nfrom unitelabs.labware.math import Decimal\n\n\ndef _ethanol_mixture() -> Mixture:\n    return Mixture({PredefinedLiquids.ETHANOL: 1})\n\n\n@dataclasses.dataclass\nclass EthanolLiquidClass(BravoLiquidClass):\n    liquid: Mixture = dataclasses.field(default_factory=lambda: Mixture({Liquid.ETHANOL: 1}))\n    tip: type[AgilentTip] = AgilentTip_250\n    min_volume: Decimal = dataclasses.field(default=Decimal(default=\"0\"))\n    max_volume: Decimal = dataclasses.field(default=Decimal(default=\"250\"))\n    coefficients: list[decimal.Decimal] = dataclasses.field(\n        default_factory=lambda: [decimal.Decimal(\"0.05\"), decimal.Decimal(\"1.02\")]\n    )\n    aspirate_velocity: Decimal = dataclasses.field(default=Decimal(default=\"35.0\"))\n    dispense_velocity: Decimal = dataclasses.field(default=Decimal(default=\"40.0\"))\n",[944,1511,1512,1519,1526,1530,1542,1553,1565,1577,1581,1585,1597,1617,1621,1625,1631,1648,1677,1687,1716,1741,1752,1775,1781,1806],{"__ignoreMap":954},[958,1513,1514,1516],{"class":960,"line":961},[958,1515,972],{"class":964},[958,1517,1518],{"class":968}," dataclasses\n",[958,1520,1521,1523],{"class":960,"line":978},[958,1522,972],{"class":964},[958,1524,1525],{"class":968}," decimal\n",[958,1527,1528],{"class":960,"line":985},[958,1529,982],{"emptyLinePlaceholder":981},[958,1531,1532,1534,1537,1539],{"class":960,"line":996},[958,1533,965],{"class":964},[958,1535,1536],{"class":968}," unitelabs.labware.agilent.liquids.bravo_liquid_class ",[958,1538,972],{"class":964},[958,1540,1541],{"class":968}," BravoLiquidClass\n",[958,1543,1544,1546,1548,1550],{"class":960,"line":1001},[958,1545,965],{"class":964},[958,1547,1174],{"class":968},[958,1549,972],{"class":964},[958,1551,1552],{"class":968}," AgilentTip_250, AgilentTip\n",[958,1554,1555,1557,1560,1562],{"class":960,"line":1008},[958,1556,965],{"class":964},[958,1558,1559],{"class":968}," unitelabs.labware.liquids ",[958,1561,972],{"class":964},[958,1563,1564],{"class":968}," Mixture, PredefinedLiquids\n",[958,1566,1567,1569,1572,1574],{"class":960,"line":1023},[958,1568,965],{"class":964},[958,1570,1571],{"class":968}," unitelabs.labware.math ",[958,1573,972],{"class":964},[958,1575,1576],{"class":968}," Decimal\n",[958,1578,1579],{"class":960,"line":1028},[958,1580,982],{"emptyLinePlaceholder":981},[958,1582,1583],{"class":960,"line":1034},[958,1584,982],{"emptyLinePlaceholder":981},[958,1586,1587,1590,1594],{"class":960,"line":1043},[958,1588,1589],{"class":964},"def",[958,1591,1593],{"class":1592},"s4qFZ"," _ethanol_mixture",[958,1595,1596],{"class":968},"() -> Mixture:\n",[958,1598,1599,1602,1605,1608,1611,1614],{"class":960,"line":1055},[958,1600,1601],{"class":964},"    return",[958,1603,1604],{"class":968}," Mixture({PredefinedLiquids.",[958,1606,1607],{"class":1019},"ETHANOL",[958,1609,1610],{"class":968},": ",[958,1612,1613],{"class":1019},"1",[958,1615,1616],{"class":968},"})\n",[958,1618,1619],{"class":960,"line":1069},[958,1620,982],{"emptyLinePlaceholder":981},[958,1622,1623],{"class":960,"line":1080},[958,1624,982],{"emptyLinePlaceholder":981},[958,1626,1628],{"class":960,"line":1627},14,[958,1629,1630],{"class":1592},"@dataclasses.dataclass\n",[958,1632,1634,1637,1640,1643,1645],{"class":960,"line":1633},15,[958,1635,1636],{"class":964},"class",[958,1638,1639],{"class":1046}," EthanolLiquidClass",[958,1641,1642],{"class":968},"(",[958,1644,1505],{"class":1019},[958,1646,1647],{"class":968},"):\n",[958,1649,1651,1654,1656,1659,1662,1665,1668,1670,1672,1674],{"class":960,"line":1650},16,[958,1652,1653],{"class":968},"    liquid: Mixture ",[958,1655,991],{"class":964},[958,1657,1658],{"class":968}," dataclasses.field(",[958,1660,1661],{"class":1046},"default_factory",[958,1663,1664],{"class":964},"=lambda",[958,1666,1667],{"class":968},": Mixture({Liquid.",[958,1669,1607],{"class":1019},[958,1671,1610],{"class":968},[958,1673,1613],{"class":1019},[958,1675,1676],{"class":968},"}))\n",[958,1678,1680,1683,1685],{"class":960,"line":1679},17,[958,1681,1682],{"class":968},"    tip: type[AgilentTip] ",[958,1684,991],{"class":964},[958,1686,1179],{"class":968},[958,1688,1690,1693,1695,1697,1700,1702,1705,1707,1709,1713],{"class":960,"line":1689},18,[958,1691,1692],{"class":968},"    min_volume: Decimal ",[958,1694,991],{"class":964},[958,1696,1658],{"class":968},[958,1698,1699],{"class":1046},"default",[958,1701,991],{"class":964},[958,1703,1704],{"class":968},"Decimal(",[958,1706,1699],{"class":1046},[958,1708,991],{"class":964},[958,1710,1712],{"class":1711},"sCx38","\"0\"",[958,1714,1715],{"class":968},"))\n",[958,1717,1719,1722,1724,1726,1728,1730,1732,1734,1736,1739],{"class":960,"line":1718},19,[958,1720,1721],{"class":968},"    max_volume: Decimal ",[958,1723,991],{"class":964},[958,1725,1658],{"class":968},[958,1727,1699],{"class":1046},[958,1729,991],{"class":964},[958,1731,1704],{"class":968},[958,1733,1699],{"class":1046},[958,1735,991],{"class":964},[958,1737,1738],{"class":1711},"\"250\"",[958,1740,1715],{"class":968},[958,1742,1744,1747,1749],{"class":960,"line":1743},20,[958,1745,1746],{"class":968},"    coefficients: list[decimal.Decimal] ",[958,1748,991],{"class":964},[958,1750,1751],{"class":968}," dataclasses.field(\n",[958,1753,1755,1758,1760,1763,1766,1769,1772],{"class":960,"line":1754},21,[958,1756,1757],{"class":1046},"        default_factory",[958,1759,1664],{"class":964},[958,1761,1762],{"class":968},": [decimal.Decimal(",[958,1764,1765],{"class":1711},"\"0.05\"",[958,1767,1768],{"class":968},"), decimal.Decimal(",[958,1770,1771],{"class":1711},"\"1.02\"",[958,1773,1774],{"class":968},")]\n",[958,1776,1778],{"class":960,"line":1777},22,[958,1779,1780],{"class":968},"    )\n",[958,1782,1784,1787,1789,1791,1793,1795,1797,1799,1801,1804],{"class":960,"line":1783},23,[958,1785,1786],{"class":968},"    aspirate_velocity: Decimal ",[958,1788,991],{"class":964},[958,1790,1658],{"class":968},[958,1792,1699],{"class":1046},[958,1794,991],{"class":964},[958,1796,1704],{"class":968},[958,1798,1699],{"class":1046},[958,1800,991],{"class":964},[958,1802,1803],{"class":1711},"\"35.0\"",[958,1805,1715],{"class":968},[958,1807,1809,1812,1814,1816,1818,1820,1822,1824,1826,1829],{"class":960,"line":1808},24,[958,1810,1811],{"class":968},"    dispense_velocity: Decimal ",[958,1813,991],{"class":964},[958,1815,1658],{"class":968},[958,1817,1699],{"class":1046},[958,1819,991],{"class":964},[958,1821,1704],{"class":968},[958,1823,1699],{"class":1046},[958,1825,991],{"class":964},[958,1827,1828],{"class":1711},"\"40.0\"",[958,1830,1715],{"class":968},[915,1832,1833,1834,1837,1838,1841],{},"The coefficients ",[944,1835,1836],{},"[0.05, 1.02]"," define a linear correction: ",[944,1839,1840],{},"corrected = 0.05 + 1.02 × volume",". Only the parameters you want to override need to be specified; all others inherit from the default values.",[949,1843,1845],{"className":951,"code":1844,"language":953,"meta":954,"style":954},"await bravo.pipette_head.aspirate(\n    plate=source_reservoir,\n    volume=100,\n    liquid_class=EthanolLiquidClass,\n)\n",[944,1846,1847,1853,1861,1871,1880],{"__ignoreMap":954},[958,1848,1849,1851],{"class":960,"line":961},[958,1850,1037],{"class":964},[958,1852,1040],{"class":968},[958,1854,1855,1857,1859],{"class":960,"line":978},[958,1856,1047],{"class":1046},[958,1858,991],{"class":964},[958,1860,1052],{"class":968},[958,1862,1863,1865,1867,1869],{"class":960,"line":985},[958,1864,1058],{"class":1046},[958,1866,991],{"class":964},[958,1868,1063],{"class":1019},[958,1870,1066],{"class":968},[958,1872,1873,1875,1877],{"class":960,"line":996},[958,1874,1072],{"class":1046},[958,1876,991],{"class":964},[958,1878,1879],{"class":968},"EthanolLiquidClass,\n",[958,1881,1882],{"class":960,"line":1001},[958,1883,1083],{"class":968},[936,1885,1887],{"id":1886},"custom-liquid-class-with-calibration-curve","Custom Liquid Class with Calibration Curve",[915,1889,1890,1891,1894,1895,1897],{},"When you have measured calibration data but don't know the exact correction formula, you can provide a ",[944,1892,1893],{},"curve"," dictionary and let the SDK fit a polynomial automatically. Set ",[944,1896,1318],{}," to an integer specifying the polynomial order.",[949,1899,1901],{"className":951,"code":1900,"language":953,"meta":954,"style":954},"@dataclasses.dataclass\nclass CalibratedEthanol(BravoLiquidClass):\n    tip: type[AgilentTip] = AgilentTip_250\n    min_volume: Decimal = dataclasses.field(default=Decimal(default=\"51\"))\n    max_volume: Decimal = dataclasses.field(default=Decimal(default=\"250\"))\n    # Set coefficients to an int to specify polynomial order for fitting\n    coefficients: list[decimal.Decimal] | int = 2\n    # Measured calibration points: target µL → corrected plunger µL\n    curve: dict[float, float] | None = dataclasses.field(\n        default_factory=lambda: {\n            0:   0.0,\n            50:  51.8,\n            100: 102.5,\n            150: 153.4,\n            200: 204.6,\n            250: 255.9,\n        }\n    )\n    aspirate_velocity: Decimal = dataclasses.field(default=Decimal(default=\"35.0\"))\n    aspirate_acceleration: Decimal = dataclasses.field(default=Decimal(default=\"75.0\"))\n    aspirate_velocity_into_wells: Decimal = dataclasses.field(default=Decimal(default=\"45.0\"))\n    aspirate_velocity_out_of_wells: Decimal = dataclasses.field(default=Decimal(default=\"55.0\"))\n    aspirate_acceleration_into_wells: Decimal = dataclasses.field(default=Decimal(default=\"90.0\"))\n    aspirate_acceleration_out_of_wells: Decimal = dataclasses.field(default=Decimal(default=\"110.0\"))\n    aspirate_post_delay_ms: int = 500\n    dispense_velocity: Decimal = dataclasses.field(default=Decimal(default=\"40.0\"))\n    dispense_acceleration: Decimal = dataclasses.field(default=Decimal(default=\"80.0\"))\n    dispense_velocity_into_wells: Decimal = dataclasses.field(default=Decimal(default=\"55.0\"))\n    dispense_velocity_out_of_wells: Decimal = dataclasses.field(default=Decimal(default=\"45.0\"))\n    dispense_acceleration_into_wells: Decimal = dataclasses.field(default=Decimal(default=\"110.0\"))\n    dispense_acceleration_out_of_wells: Decimal = dataclasses.field(default=Decimal(default=\"90.0\"))\n    dispense_post_delay_ms: int = 500\n",[944,1902,1903,1907,1920,1928,1951,1973,1978,1994,1999,2024,2033,2046,2059,2071,2083,2095,2107,2112,2116,2138,2162,2186,2210,2234,2258,2272,2295,2320,2344,2368,2392,2416],{"__ignoreMap":954},[958,1904,1905],{"class":960,"line":961},[958,1906,1630],{"class":1592},[958,1908,1909,1911,1914,1916,1918],{"class":960,"line":978},[958,1910,1636],{"class":964},[958,1912,1913],{"class":1046}," CalibratedEthanol",[958,1915,1642],{"class":968},[958,1917,1505],{"class":1019},[958,1919,1647],{"class":968},[958,1921,1922,1924,1926],{"class":960,"line":985},[958,1923,1682],{"class":968},[958,1925,991],{"class":964},[958,1927,1179],{"class":968},[958,1929,1930,1932,1934,1936,1938,1940,1942,1944,1946,1949],{"class":960,"line":996},[958,1931,1692],{"class":968},[958,1933,991],{"class":964},[958,1935,1658],{"class":968},[958,1937,1699],{"class":1046},[958,1939,991],{"class":964},[958,1941,1704],{"class":968},[958,1943,1699],{"class":1046},[958,1945,991],{"class":964},[958,1947,1948],{"class":1711},"\"51\"",[958,1950,1715],{"class":968},[958,1952,1953,1955,1957,1959,1961,1963,1965,1967,1969,1971],{"class":960,"line":1001},[958,1954,1721],{"class":968},[958,1956,991],{"class":964},[958,1958,1658],{"class":968},[958,1960,1699],{"class":1046},[958,1962,991],{"class":964},[958,1964,1704],{"class":968},[958,1966,1699],{"class":1046},[958,1968,991],{"class":964},[958,1970,1738],{"class":1711},[958,1972,1715],{"class":968},[958,1974,1975],{"class":960,"line":1008},[958,1976,1977],{"class":1004},"    # Set coefficients to an int to specify polynomial order for fitting\n",[958,1979,1980,1982,1985,1988,1991],{"class":960,"line":1023},[958,1981,1746],{"class":968},[958,1983,1984],{"class":964},"|",[958,1986,1987],{"class":1019}," int",[958,1989,1990],{"class":964}," =",[958,1992,1993],{"class":1019}," 2\n",[958,1995,1996],{"class":960,"line":1028},[958,1997,1998],{"class":1004},"    # Measured calibration points: target µL → corrected plunger µL\n",[958,2000,2001,2004,2007,2010,2012,2015,2017,2020,2022],{"class":960,"line":1034},[958,2002,2003],{"class":968},"    curve: dict[",[958,2005,2006],{"class":1019},"float",[958,2008,2009],{"class":968},", ",[958,2011,2006],{"class":1019},[958,2013,2014],{"class":968},"] ",[958,2016,1984],{"class":964},[958,2018,2019],{"class":1019}," None",[958,2021,1990],{"class":964},[958,2023,1751],{"class":968},[958,2025,2026,2028,2030],{"class":960,"line":1043},[958,2027,1757],{"class":1046},[958,2029,1664],{"class":964},[958,2031,2032],{"class":968},": {\n",[958,2034,2035,2038,2041,2044],{"class":960,"line":1055},[958,2036,2037],{"class":1019},"            0",[958,2039,2040],{"class":968},":   ",[958,2042,2043],{"class":1019},"0.0",[958,2045,1066],{"class":968},[958,2047,2048,2051,2054,2057],{"class":960,"line":1069},[958,2049,2050],{"class":1019},"            50",[958,2052,2053],{"class":968},":  ",[958,2055,2056],{"class":1019},"51.8",[958,2058,1066],{"class":968},[958,2060,2061,2064,2066,2069],{"class":960,"line":1080},[958,2062,2063],{"class":1019},"            100",[958,2065,1610],{"class":968},[958,2067,2068],{"class":1019},"102.5",[958,2070,1066],{"class":968},[958,2072,2073,2076,2078,2081],{"class":960,"line":1627},[958,2074,2075],{"class":1019},"            150",[958,2077,1610],{"class":968},[958,2079,2080],{"class":1019},"153.4",[958,2082,1066],{"class":968},[958,2084,2085,2088,2090,2093],{"class":960,"line":1633},[958,2086,2087],{"class":1019},"            200",[958,2089,1610],{"class":968},[958,2091,2092],{"class":1019},"204.6",[958,2094,1066],{"class":968},[958,2096,2097,2100,2102,2105],{"class":960,"line":1650},[958,2098,2099],{"class":1019},"            250",[958,2101,1610],{"class":968},[958,2103,2104],{"class":1019},"255.9",[958,2106,1066],{"class":968},[958,2108,2109],{"class":960,"line":1679},[958,2110,2111],{"class":968},"        }\n",[958,2113,2114],{"class":960,"line":1689},[958,2115,1780],{"class":968},[958,2117,2118,2120,2122,2124,2126,2128,2130,2132,2134,2136],{"class":960,"line":1718},[958,2119,1786],{"class":968},[958,2121,991],{"class":964},[958,2123,1658],{"class":968},[958,2125,1699],{"class":1046},[958,2127,991],{"class":964},[958,2129,1704],{"class":968},[958,2131,1699],{"class":1046},[958,2133,991],{"class":964},[958,2135,1803],{"class":1711},[958,2137,1715],{"class":968},[958,2139,2140,2143,2145,2147,2149,2151,2153,2155,2157,2160],{"class":960,"line":1743},[958,2141,2142],{"class":968},"    aspirate_acceleration: Decimal ",[958,2144,991],{"class":964},[958,2146,1658],{"class":968},[958,2148,1699],{"class":1046},[958,2150,991],{"class":964},[958,2152,1704],{"class":968},[958,2154,1699],{"class":1046},[958,2156,991],{"class":964},[958,2158,2159],{"class":1711},"\"75.0\"",[958,2161,1715],{"class":968},[958,2163,2164,2167,2169,2171,2173,2175,2177,2179,2181,2184],{"class":960,"line":1754},[958,2165,2166],{"class":968},"    aspirate_velocity_into_wells: Decimal ",[958,2168,991],{"class":964},[958,2170,1658],{"class":968},[958,2172,1699],{"class":1046},[958,2174,991],{"class":964},[958,2176,1704],{"class":968},[958,2178,1699],{"class":1046},[958,2180,991],{"class":964},[958,2182,2183],{"class":1711},"\"45.0\"",[958,2185,1715],{"class":968},[958,2187,2188,2191,2193,2195,2197,2199,2201,2203,2205,2208],{"class":960,"line":1777},[958,2189,2190],{"class":968},"    aspirate_velocity_out_of_wells: Decimal ",[958,2192,991],{"class":964},[958,2194,1658],{"class":968},[958,2196,1699],{"class":1046},[958,2198,991],{"class":964},[958,2200,1704],{"class":968},[958,2202,1699],{"class":1046},[958,2204,991],{"class":964},[958,2206,2207],{"class":1711},"\"55.0\"",[958,2209,1715],{"class":968},[958,2211,2212,2215,2217,2219,2221,2223,2225,2227,2229,2232],{"class":960,"line":1783},[958,2213,2214],{"class":968},"    aspirate_acceleration_into_wells: Decimal ",[958,2216,991],{"class":964},[958,2218,1658],{"class":968},[958,2220,1699],{"class":1046},[958,2222,991],{"class":964},[958,2224,1704],{"class":968},[958,2226,1699],{"class":1046},[958,2228,991],{"class":964},[958,2230,2231],{"class":1711},"\"90.0\"",[958,2233,1715],{"class":968},[958,2235,2236,2239,2241,2243,2245,2247,2249,2251,2253,2256],{"class":960,"line":1808},[958,2237,2238],{"class":968},"    aspirate_acceleration_out_of_wells: Decimal ",[958,2240,991],{"class":964},[958,2242,1658],{"class":968},[958,2244,1699],{"class":1046},[958,2246,991],{"class":964},[958,2248,1704],{"class":968},[958,2250,1699],{"class":1046},[958,2252,991],{"class":964},[958,2254,2255],{"class":1711},"\"110.0\"",[958,2257,1715],{"class":968},[958,2259,2261,2264,2267,2269],{"class":960,"line":2260},25,[958,2262,2263],{"class":968},"    aspirate_post_delay_ms: ",[958,2265,2266],{"class":1019},"int",[958,2268,1990],{"class":964},[958,2270,2271],{"class":1019}," 500\n",[958,2273,2275,2277,2279,2281,2283,2285,2287,2289,2291,2293],{"class":960,"line":2274},26,[958,2276,1811],{"class":968},[958,2278,991],{"class":964},[958,2280,1658],{"class":968},[958,2282,1699],{"class":1046},[958,2284,991],{"class":964},[958,2286,1704],{"class":968},[958,2288,1699],{"class":1046},[958,2290,991],{"class":964},[958,2292,1828],{"class":1711},[958,2294,1715],{"class":968},[958,2296,2298,2301,2303,2305,2307,2309,2311,2313,2315,2318],{"class":960,"line":2297},27,[958,2299,2300],{"class":968},"    dispense_acceleration: Decimal ",[958,2302,991],{"class":964},[958,2304,1658],{"class":968},[958,2306,1699],{"class":1046},[958,2308,991],{"class":964},[958,2310,1704],{"class":968},[958,2312,1699],{"class":1046},[958,2314,991],{"class":964},[958,2316,2317],{"class":1711},"\"80.0\"",[958,2319,1715],{"class":968},[958,2321,2323,2326,2328,2330,2332,2334,2336,2338,2340,2342],{"class":960,"line":2322},28,[958,2324,2325],{"class":968},"    dispense_velocity_into_wells: Decimal ",[958,2327,991],{"class":964},[958,2329,1658],{"class":968},[958,2331,1699],{"class":1046},[958,2333,991],{"class":964},[958,2335,1704],{"class":968},[958,2337,1699],{"class":1046},[958,2339,991],{"class":964},[958,2341,2207],{"class":1711},[958,2343,1715],{"class":968},[958,2345,2347,2350,2352,2354,2356,2358,2360,2362,2364,2366],{"class":960,"line":2346},29,[958,2348,2349],{"class":968},"    dispense_velocity_out_of_wells: Decimal ",[958,2351,991],{"class":964},[958,2353,1658],{"class":968},[958,2355,1699],{"class":1046},[958,2357,991],{"class":964},[958,2359,1704],{"class":968},[958,2361,1699],{"class":1046},[958,2363,991],{"class":964},[958,2365,2183],{"class":1711},[958,2367,1715],{"class":968},[958,2369,2371,2374,2376,2378,2380,2382,2384,2386,2388,2390],{"class":960,"line":2370},30,[958,2372,2373],{"class":968},"    dispense_acceleration_into_wells: Decimal ",[958,2375,991],{"class":964},[958,2377,1658],{"class":968},[958,2379,1699],{"class":1046},[958,2381,991],{"class":964},[958,2383,1704],{"class":968},[958,2385,1699],{"class":1046},[958,2387,991],{"class":964},[958,2389,2255],{"class":1711},[958,2391,1715],{"class":968},[958,2393,2395,2398,2400,2402,2404,2406,2408,2410,2412,2414],{"class":960,"line":2394},31,[958,2396,2397],{"class":968},"    dispense_acceleration_out_of_wells: Decimal ",[958,2399,991],{"class":964},[958,2401,1658],{"class":968},[958,2403,1699],{"class":1046},[958,2405,991],{"class":964},[958,2407,1704],{"class":968},[958,2409,1699],{"class":1046},[958,2411,991],{"class":964},[958,2413,2231],{"class":1711},[958,2415,1715],{"class":968},[958,2417,2419,2422,2424,2426],{"class":960,"line":2418},32,[958,2420,2421],{"class":968},"    dispense_post_delay_ms: 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